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How to Self-Inject Peptides
Your provider has prescribed a peptide that you will inject at home. This guide walks you through the process step by step. If this is your first time, your provider or nurse will demonstrate the injection technique before you do it on your own. Do not hesitate to ask questions.
Supplies You Will Need
Gather all supplies before you begin. You will need:
Step-by-Step Injection Guide
  1. Wash your hands thoroughly with soap and warm water for at least 20 seconds. Dry with a clean towel.
  2. Gather your supplies and place them on a clean, flat surface. Check the expiration date on your medication. Look at the liquid — it should be clear and free of particles or cloudiness.
  3. Clean the vial top with a fresh alcohol swab. Let it air dry for a few seconds.
  4. Draw up your medication. Remove the syringe cap. Pull back the plunger to draw in air equal to your dose. Insert the needle into the vial, push in the air, then turn the vial upside down. Pull the plunger back to fill the syringe to your prescribed dose. Tap the syringe gently and push out any air bubbles. (Your provider will demonstrate this technique.)
  5. Choose your injection site and clean it with a fresh alcohol swab. Let the area air dry completely. See the injection site rotation section below.
  6. Pinch the skin at the injection site between your thumb and forefinger. This lifts the fatty tissue away from the muscle.
  7. Insert the needle at a 45 to 90 degree angle (your provider will tell you which angle to use). Use a quick, smooth motion — like throwing a dart.
  8. Inject the medication slowly by pushing the plunger down with steady pressure. Take about 5 seconds.
  9. Wait 5 to 10 seconds before removing the needle. This helps ensure all the medication is delivered.
  10. Remove the needle and release the pinched skin. Apply gentle pressure with a gauze pad or cotton ball if needed. Do not rub the injection site.
  11. Dispose of the needle immediately in your sharps container. Never recap a used needle. Never throw loose needles in the trash.
Injection Site Rotation
Rotate your injection site each time to prevent irritation and lumps under the skin. Common injection areas include: Tip: Move at least 1 inch from your last injection site. You can rotate clockwise around your belly, alternate thighs, or switch between areas each day.
Injection Site Rotation Diagram

Abdomen (most common): Imagine a clock around your belly button. Inject at 12, 3, 6, and 9 o'clock positions, staying at least 2 inches from the navel.


Thighs: Front or outer upper thigh. Alternate left and right.


Upper arms: Fleshy back portion of the upper arm. Alternate left and right.

What Is Normal After an Injection
After your injection, it is normal to see: These are all normal and do not mean anything went wrong.
When to Call Your Provider
Contact your provider if you notice:
Sharps Disposal
Used needles and syringes must be disposed of safely:
Quick Reference Checklist